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CoraLite®555-conjugated Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

Cat No : CL555-13511

Print datasheet

Synonyms

B2M, beta 2 microglobulin, Beta-2-Microglobulin, HDCMA22P

验证数据展示

  • IF Staining of A431 using CL555-13511

    IF Staining of A431 using CL555-13511

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®555 Beta-2-Microglobulin antibody (CL555-13511) at dilution of 1:200.

  • FC experiment of HeLa using CL555-13511

    FC experiment of HeLa using CL555-13511

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Beta-2-Microglobulin (CL555-13511) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inA431 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13511-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-13511 targets Beta-2-Microglobulin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse, rat
Immunogen Beta-2-Microglobulin fusion protein Ag4433 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name beta-2-microglobulin
Synonyms B2M, beta 2 microglobulin, Beta-2-Microglobulin, HDCMA22P
Calculated Molecular Weight 119 aa, 14 kDa
Observed Molecular Weight 12-14 kDa
GenBank Accession NumberBC032589
Gene Symbol B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
RRIDAB_2919616
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61769
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

实验方案

Product Specific Protocols
IF protocol for CL555 Beta-2-Microglobulin antibody CL555-13511Download protocol
Standard Protocols
Click here to view our Standard Protocols