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验证数据展示

  • IF Staining of A431 using CL555-13511

    IF Staining of A431 using CL555-13511

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®555 Beta-2-Microglobulin antibody (CL555-13511) at dilution of 1:200.

CoraLite®555-conjugated Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for IF/ICC
Cat No. CL555-13511

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

B2M, beta 2 microglobulin, Beta-2-Microglobulin, HDCMA22P

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy Beta-2-Microglobulin Ready-To-Use IHC Kit. Beta-2-Microglobulin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13511-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-13511 targets Beta-2-Microglobulin in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 Beta-2-Microglobulin fusion protein Ag4433 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 beta-2-microglobulin
别名 B2M, beta 2 microglobulin, Beta-2-Microglobulin, HDCMA22P
计算分子量 119 aa, 14 kDa
观测分子量 12-14 kDa
GenBank蛋白编号BC032589
基因名称 B2M
Gene ID (NCBI) 567
ENSEMBL Gene IDENSG00000166710
RRIDAB_2919616
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61769
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

实验方案

Product Specific Protocols
IF protocol for CL555 Beta-2-Microglobulin antibody CL555-13511Download protocol
Standard Protocols
Click here to view our Standard Protocols