Beta Catenin Recombinant antibody

Beta Catenin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 80488-1-RR

产品说明书

CloneNo. 2J24

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig, zebrafish

应用

WB, IHC, IF/ICC, ELISA

2J24, b cat, B-catenin, beta-Catenin, Catenin beta 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, zebrafish tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human colon cancer tissue, human rectal cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80488-1-RR targets Beta Catenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, zebrafish
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 2J24, b cat, B-catenin, beta-Catenin, Catenin beta 1
计算分子量 781 aa, 86 kDa
观测分子量92 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2918895
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

实验方案

Product Specific Protocols
WB protocol for Beta Catenin antibody 80488-1-RRDownload protocol
IHC protocol for Beta Catenin antibody 80488-1-RRDownload protocol
IF protocol for Beta Catenin antibody 80488-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...