验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, zebrafish tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue, human colon cancer tissue, human rectal cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80488-1-RR targets Beta Catenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat, pig, zebrafish |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | catenin (cadherin-associated protein), beta 1, 88kDa |
别名 | 2J24, b cat, B-catenin, beta-Catenin, Catenin beta 1 |
计算分子量 | 781 aa, 86 kDa |
观测分子量 | 92 kDa |
GenBank蛋白编号 | BC058926 |
基因名称 | Beta Catenin |
Gene ID (NCBI) | 1499 |
ENSEMBL Gene ID | ENSG00000168036 |
RRID | AB_2918895 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P35222 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
实验方案
Product Specific Protocols | |
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WB protocol for Beta Catenin antibody 80488-1-RR | Download protocol |
IHC protocol for Beta Catenin antibody 80488-1-RR | Download protocol |
IF protocol for Beta Catenin antibody 80488-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |