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验证数据展示

  • IF Staining of MCF-7 using CL488-80488

    IF Staining of MCF-7 using CL488-80488

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 Beta Catenin antibody (CL488-80488, Clone: 2J24 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Beta Catenin Recombinant antibody

Beta Catenin Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-80488

产品说明书

CloneNo. 2J24

宿主/亚型

Rabbit / IgG

种属反应性

Human, Pig, Mouse, Rat, Zebrafish

应用

IF/ICC

别名

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80488-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80488 targets Beta Catenin in IF/ICC applications and shows reactivity with Human, Pig, Mouse, Rat, Zebrafish samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Pig, Mouse, Rat, Zebrafish
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, PRO2286
计算分子量 781 aa, 86 kDa
观测分子量92 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2934628
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35222
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin is a 92-kDa protein, originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Beta Catenin antibody CL488-80488Download protocol
Standard Protocols
Click here to view our Standard Protocols