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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BubR1 Polyclonal antibody

BubR1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-11504

Print datasheet

Synonyms

Bub1A, BUB1B, BUB1beta, BUBR1, hBUBR1, MAD3L, Protein SSK1, SSK1

验证数据展示

  • IF Staining of SKOV-3 using CL488-11504

    IF Staining of SKOV-3 using CL488-11504

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 488 BubR1 antibody (CL488-11504) at dilution of 1:100.

经过测试的应用

Positive IF detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy BUB1B Ready-To-Use IHC Kit. BUB1B primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11504 targets BubR1 in IF applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen BubR1 fusion protein Ag2053 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Synonyms Bub1A, BUB1B, BUB1beta, BUBR1, hBUBR1, MAD3L, Protein SSK1, SSK1
Calculated Molecular Weight 1050 aa, 120 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC018739
Gene Symbol BUB1B
Gene ID (NCBI) 701
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60566
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BubR1 antibody CL488-11504Download protocol
Standard Protocols
Click here to view our Standard Protocols