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C12orf29 Recombinant antibody, PBS Only (Capture)

C12orf29 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242163C3

Cat no : 84708-1-PBS

Print datasheet

Synonyms

Rnl, RNA Ligase 1, RNA Ligase, 242163C3

验证数据展示

  • WB analysis of HeLa using 84708-1-RR (same clone as 84708-1-PBS)

    WB analysis of HeLa using 84708-1-RR (same clone as 84708-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84708-1-RR (C12orf29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84708-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01512-1

    Cytometric bead array standard curve of MP01512-1

    Cytometric bead array standard curve of MP01512-1, C12orf29 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84708-1-PBS. Detection antibody: 84708-2-PBS. Standard: Ag35681. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84708-1-RR

    Affinity and Kinetic Characterization of 84708-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84708-1-RR against Human C12orf29 were performed. The affinity constant is below 1 pM.

产品信息

84708-1-PBS targets C12orf29 as part of a matched antibody pair:

MP01512-1: 84708-1-PBS capture and 84708-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen C12orf29 fusion protein Ag35681 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 12 open reading frame 29
Synonyms Rnl, RNA Ligase 1, RNA Ligase, 242163C3
Calculated Molecular Weight37kDa，325aa
Observed Molecular Weight37 kDa
GenBank Accession NumberNM_001009894.2
Gene Symbol C12orf29
Gene ID (NCBI) 91298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N999
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).