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验证数据展示

  • IF Staining of HepG2 using CL488-67063

    IF Staining of HepG2 using CL488-67063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 C1QA antibody (CL488-67063, Clone: 1B10E3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated C1QA Monoclonal antibody

C1QA Monoclonal Antibody for IF/ICC
Cat No. CL488-67063

产品说明书

CloneNo. 1B10E3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC

别名

C1QA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy C1QA Ready-To-Use IHC Kit. C1QA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67063-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67063 targets C1QA in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 C1QA fusion protein Ag28622 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component 1, q subcomponent, A chain
别名 C1QA
计算分子量 245 aa, 26 kDa
观测分子量 30 kDa
GenBank蛋白编号BC030153
基因名称 C1QA
Gene ID (NCBI) 712
RRIDAB_2919415
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02745
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C1QA antibody CL488-67063Download protocol
Standard Protocols
Click here to view our Standard Protocols