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CoraLite®594-conjugated C1QA Monoclonal antibody

C1QA Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B10E3

Cat no : CL594-67063

Print datasheet

Synonyms

C1QA

验证数据展示

  • IF Staining of HepG2 using CL594-67063

    IF Staining of HepG2 using CL594-67063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 C1QA antibody (CL594-67063, Clone: 1B10E3 ) at dilution of 1:200, CL488-Phalloidin (green).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy C1QA Ready-To-Use IHC Kit. C1QA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67063-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67063 targets C1QA in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen C1QA fusion protein Ag28622 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement component 1, q subcomponent, A chain
Synonyms C1QA
Calculated Molecular Weight 245 aa, 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC030153
Gene Symbol C1QA
Gene ID (NCBI) 712
RRIDAB_2920081
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02745
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

实验方案

Product Specific Protocols
IF protocol for CL594 C1QA antibody CL594-67063Download protocol
Standard Protocols
Click here to view our Standard Protocols