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Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody

C5AR1/CD88 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241712D4

Cat no : 98226-1-RR

Print datasheet

Synonyms

C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98226-1-RR

    FC experiment of human peripheral blood leukocytes using 98226-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR, Clone: 241712D4) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98226-1-RR

    FC experiment of human peripheral blood leukocytes using 98226-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR, Clone: 241712D4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy C5AR1 Ready-To-Use IHC Kit. C5AR1 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98226-1-RR targets C5AR1/CD88 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name complement component 5a receptor 1
Synonyms C5AR, C5AR1, C5a-R, C5a R, C5a anaphylatoxin chemotactic receptor 1
Calculated Molecular Weight39kDa
GenBank Accession NumberNM_001736.4
Gene Symbol C5aR
Gene ID (NCBI) 728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21730
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production.

实验方案

Product Specific Protocols
FC protocol for C5AR1/CD88 antibody 98226-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols