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C5orf24 Polyclonal antibody

C5orf24 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 24319-1-AP

Print datasheet

Synonyms

C5orf24, UPF0461 protein C5orf24

验证数据展示

  • WB analysis of HEK-293 using 24319-1-AP

    WB analysis of HEK-293 using 24319-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24319-1-AP (C5orf24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 24319-1-AP

    IHC staining of human pancreas using 24319-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293 using 24319-1-AP

    IP experiment of HEK-293 using 24319-1-AP

    IP result of anti-C5orf24 (IP:24319-1-AP, 4ug; Detection:24319-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

  • IF Staining of HEK-293 using 24319-1-AP

    IF Staining of HEK-293 using 24319-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C5orf24 antibody (24319-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HEK-293 using 24319-1-AP

    FC experiment of HEK-293 using 24319-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug C5orf24 Polyclonal antibody (24319-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman pancreas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

24319-1-AP targets C5orf24 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitypig
Immunogen C5orf24 fusion protein Ag19534 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 5 open reading frame 24
Synonyms C5orf24, UPF0461 protein C5orf24
Calculated Molecular Weight 188 aa, 20 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC053677
Gene Symbol C5orf24
Gene ID (NCBI) 134553
RRIDAB_2879491
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6I8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C5orf24 has two isoforms with MW 20 kDa and 16 kDa. For phosphoserine modification, the MW is about 20-23 kDa.

实验方案

Product Specific Protocols
WB protocol for C5orf24 antibody 24319-1-APDownload protocol
IHC protocol for C5orf24 antibody 24319-1-APDownload protocol
IF protocol for C5orf24 antibody 24319-1-APDownload protocol
IP protocol for C5orf24 antibody 24319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Front Immunol

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Authors - Xu Ye
View Article