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C7 Monoclonal antibody

C7 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H9C4

Cat no : 66908-1-Ig

Print datasheet

Synonyms

Complement component C7, complement component 7, 1H9C4

验证数据展示

  • WB analysis of human plasma using 66908-1-Ig

    WB analysis of human plasma using 66908-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66908-1-Ig (C7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66908-1-Ig

    IF Staining of HepG2 using 66908-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C7 antibody (66908-1-Ig, Clone: 1H9C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • FC experiment of HepG2 using 66908-1-Ig

    FC experiment of HepG2 using 66908-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug C7 Monoclonal antibody (66908-1-Ig, Clone:1H9C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66908-1-Ig targets C7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen C7 fusion protein Ag12552 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement component 7
Synonyms Complement component C7, complement component 7, 1H9C4
Calculated Molecular Weight 843 aa, 94 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC063851
Gene Symbol C7
Gene ID (NCBI) 730
RRIDAB_2882235
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C7, a single-chain plasma glycoprotein, is a component of the complement system. It is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. People with C7 deficiency are prone to bacterial infection.

实验方案

Product Specific Protocols
WB protocol for C7 antibody 66908-1-IgDownload protocol
IF protocol for C7 antibody 66908-1-IgDownload protocol
FC protocol for C7 antibody 66908-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols