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CoraLite® Plus 488-conjugated C7 Monoclonal antibody

C7 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H9C4

Cat no : CL488-66908

Print datasheet

Synonyms

Complement component C7, complement component 7, 1H9C4

验证数据展示

  • IF Staining of HepG2 using CL488-66908

    IF Staining of HepG2 using CL488-66908

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 C7 antibody (CL488-66908, Clone: 1H9C4 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66908

    FC experiment of HepG2 using CL488-66908

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated C7 Monoclonal antibody (CL488-66908, Clone:1H9C4)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66908-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66908 targets C7 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen C7 fusion protein Ag12552 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name complement component 7
Synonyms Complement component C7, complement component 7, 1H9C4
Calculated Molecular Weight 843 aa, 94 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC063851
Gene Symbol C7
Gene ID (NCBI) 730
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10643
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C7, a single-chain plasma glycoprotein, is a component of the complement system. It is a constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. People with C7 deficiency are prone to bacterial infection.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C7 antibody CL488-66908Download protocol
FC protocol for CL Plus 488 C7 antibody CL488-66908Download protocol
Standard Protocols
Click here to view our Standard Protocols