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Caspase 3/p17/p19 Polyclonal antibody

Caspase 3/p17/p19 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

FC, IF, IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19677-1-AP

Print datasheet

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

验证数据展示

  • WB analysis of Jurkat using 19677-1-AP

    WB analysis of Jurkat using 19677-1-AP

    WB result of Caspase 3 antibody (19677-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected Jurkat cells.

  • WB analysis using 19677-1-AP

    WB analysis using 19677-1-AP

    Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 19677-1-AP

    IP experiment of NIH/3T3 using 19677-1-AP

    IP result of anti-Caspase 3 (IP:19677-1-AP, 4ug; Detection:19677-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug.

  • IHC staining of mouse brain using 19677-1-AP

    IHC staining of mouse brain using 19677-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 19677-1-AP

    IHC staining of mouse brain using 19677-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 19677-1-AP

    FC experiment of HepG2 using 19677-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Caspase 3/p17/p19 (19677-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

  • IF Staining of NIH/3T3 using 19677-1-AP

    IF Staining of NIH/3T3 using 19677-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inmouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells, mouse brain tissue, HeLa cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

19677-1-AP targets Caspase 3/p17/p19 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB,ELISA
Cited Applications ELISA, IF, IHC, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Astragalus membranaceus, Bovine, canine, chicken, duck, Goat, hamster, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight 32 kDa
Observed molecular weight 32-35 kDa, 17 kDa, 19 kDa
GenBank accession numberNM_004346
Gene symbol CASP3
UniprotP42574
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

实验方案

Product Specific Protocols
WB protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
IHC protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
IF protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
IP protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
FC protocol for Caspase 3/p17/p19 antibody 19677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cancer

Lyn Kinase Promotes the Proliferation of Malignant Melanoma Cells through Inhibition of Apoptosis and Autophagy via the PI3K/Akt Signaling Pathway.

Authors - Qianqian Zhang
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mouseWB

Cell Death Dis

Suppressing the Na+/H+ exchanger 1: a new sight to treat depression.

Authors - Xueyang Deng
View Article
mouseWB,IHC

Int Immunopharmacol

Protective effects of levo-tetrahydropalmatine on hepatic ischemia/reperfusion injury are mediated by inhibition of the ERK/NF-κB pathway.

Authors - Qiang Yu
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humanWB

Onco Targets Ther

XRCC1 rs1799782 (C194T) polymorphism correlated with tumor metastasis and molecular subtypes in breast cancer.

Authors - Qing Li
View Article
humanWB

Lipids Health Dis

κ-Opioid receptor stimulation reduces palmitate-induced apoptosis via Akt/eNOS signaling pathway.

Authors - Yan Cui
View Article
humanWB,IHC

Invest New Drugs

Identification of a 3,3-difluorinated tetrahydropyridinol compound as a novel antitumor agent for hepatocellular carcinoma acting via cell cycle arrest through disturbing CDK7-mediated phosphorylation of Cdc2.

Authors - Wu Bi
View Article