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Caspase 3/p17/p19 Polyclonal antibody 1036 Publications

Rabbit Polyclonal| Catalog number: 19677-1-AP

Featured Product KD/KO validated

浓度: 600 μg/ml

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Applications

Tested Applications:
FC, IF, IHC, IP, WB,ELISA
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Cited Applications:
ELISA, IF, IHC, RIP, WB


Images See all

WB analysis using 19677-1-AP

Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

WB analysis of Jurkat using 19677-1-AP

WB result of Caspase 3 antibody (19677-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected Jurkat cells.

IP experiment of NIH/3T3 using 19677-1-AP

IP result of anti-Caspase 3 (IP:19677-1-AP, 4ug; Detection:19677-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug.

IHC staining of mouse brain using 19677-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC staining of mouse brain using 19677-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19677-1-AP (Caspase 3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FC experiment of HepG2 using 19677-1-AP

1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Caspase 3/p17/p19 (19677-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

IF Staining of NIH/3T3 using 19677-1-AP

Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Species Specificity:
human, mouse, rat
Cited Species:
Astragalus membranaceus, Bovine, chicken, duck, Goat, hamster, human, monkey, mouse, pig
Positive WB detected in:
Jurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue
Positive IP detected in:
NIH/3T3 cells
Positive IHC detected in:
mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
NIH/3T3 cells, mouse brain tissue, HeLa cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight:
32 kDa
Observed molecular weight:
32-35 kDa, 17 kDa, 19 kDa
GenBank accession number:
NM_004346
Gene ID (NCBI):
836
Gene symbol
CASP3
Synonyms
Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Background

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

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