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- KD/KO Validated
Caspase 3/p17/p19 Polyclonal antibody
Caspase 3/p17/p19 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (8)
Applications
FC, IF, IHC, IP, WB,ELISA and More (1)
Conjugate
Unconjugated
1285
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue |
Positive IP detected in | NIH/3T3 cells |
Positive IHC detected in | mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells, mouse brain tissue, HeLa cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
产品信息
19677-1-AP targets Caspase 3/p17/p19 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB,ELISA |
Cited Applications | ELISA, IF, IHC, RIP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, Astragalus membranaceus, Bovine, canine, chicken, duck, Goat, hamster, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | caspase 3, apoptosis-related cysteine peptidase |
Calculated molecular weight | 32 kDa |
Observed molecular weight | 32-35 kDa, 17 kDa, 19 kDa |
GenBank accession number | NM_004346 |
Gene symbol | CASP3 |
Uniprot | P42574 | Gene ID (NCBI) | 836 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
IHC protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
IF protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
IP protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
FC protocol for Caspase 3/p17/p19 antibody 19677-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Cancer Lyn Kinase Promotes the Proliferation of Malignant Melanoma Cells through Inhibition of Apoptosis and Autophagy via the PI3K/Akt Signaling Pathway. | ||
Int Immunopharmacol Protective effects of levo-tetrahydropalmatine on hepatic ischemia/reperfusion injury are mediated by inhibition of the ERK/NF-κB pathway. | ||
Onco Targets Ther XRCC1 rs1799782 (C194T) polymorphism correlated with tumor metastasis and molecular subtypes in breast cancer. | ||
Lipids Health Dis κ-Opioid receptor stimulation reduces palmitate-induced apoptosis via Akt/eNOS signaling pathway. | ||
Invest New Drugs Identification of a 3,3-difluorinated tetrahydropyridinol compound as a novel antitumor agent for hepatocellular carcinoma acting via cell cycle arrest through disturbing CDK7-mediated phosphorylation of Cdc2. |