- Featured Product
- KD/KO Validated
Caspase 3/p17/p19 Polyclonal antibody
Caspase 3/p17/p19 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Cat No : 19677-1-AP
|Positive WB detected in||Jurkat cells, mouse spleen tissue, HeLa cells, rat brain tissue, rat liver tissue|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||mouse brain tissue, human teeth tissue, human spleen tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells, mouse brain tissue, HeLa cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
19677-1-AP targets Caspase 3/p17/p19 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Applications||FC, IF, IHC, IP, WB,ELISA|
|Cited Applications||ELISA, IF, IHC, IP, RIP, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Bovine, canine, chicken, duck, Goat, hamster, monkey, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||caspase 3, apoptosis-related cysteine peptidase|
|Synonyms||Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1|
|Calculated molecular weight||32 kDa|
|Observed molecular weight||32-35 kDa, 17 kDa, 19 kDa|
|GenBank accession number||NM_004346|
|Gene ID (NCBI)||836|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
|Product Specific Protocols|
|WB protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|IHC protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|IF protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|IP protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|FC protocol for Caspase 3/p17/p19 antibody 19677-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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