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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Caspase 3/p17/p19 Polyclonal antibody

Caspase 3/p17/p19 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-19677

Print datasheet

Synonyms

CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3

验证数据展示

  • IF Staining of NIH/3T3 using CL488-19677

    IF Staining of NIH/3T3 using CL488-19677

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Caspase 3/p17/p19 antibody (CL488-19677) at dilution of 1:100.

  • IF Staining of HeLa using CL488-19677

    IF Staining of HeLa using CL488-19677

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Caspase 3/p17/p19 antibody (CL488-19677) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells, NIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 19677-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19677 targets Caspase 3/p17/p19 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase 3, apoptosis-related cysteine peptidase
Synonyms CASP3, Caspase3, Caspase-3, Caspase 3, CASP-3
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32-35 kDa, 17 kDa, 19 kDa
GenBank Accession NumberNM_004346
Gene Symbol Caspase 3
Gene ID (NCBI) 836
RRIDAB_2919166
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42574
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Caspase 3/p17/p19 antibody CL488-19677Download protocol
Standard Protocols
Click here to view our Standard Protocols