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CoraLite®594-conjugated Caspase 3/p17/p19 Polyclonal antibody

Caspase 3/p17/p19 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra), IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-19677

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Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17/p19, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1



经过测试的应用

Positive IF detected inNIH/3T3 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 19677-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-19677 targets Caspase 3/p17/p19 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra), IF
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caspase 3, apoptosis-related cysteine peptidase
Synonyms Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17/p19, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32-35 kDa, 17 kDa, 19 kDa
GenBank Accession NumberNM_004346
Gene Symbol CASP3
Gene ID (NCBI) 836
RRIDAB_2919853
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42574
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form a heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19, and p32 of Caspase 3.

实验方案

Product Specific Protocols
IF protocol for CL594 Caspase 3/p17/p19 antibody CL594-19677Download protocol
FC protocol for CL594 Caspase 3/p17/p19 antibody CL594-19677Download protocol
Standard Protocols
Click here to view our Standard Protocols