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CoraLite® Plus 488-conjugated CASQ2 Recombinant antibody

CASQ2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241211A3

Cat no : CL488-84056-6

Print datasheet

Synonyms

Calsequestrin-2, Calsequestrin 2, 241211A3

验证数据展示

  • IF Staining of C2C12 using CL488-84056-6

    IF Staining of C2C12 using CL488-84056-6

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CoraLite® Plus 488 CASQ2 antibody (CL488-84056-6, Clone: 241211A3 ) at dilution of 1:200.

  • FC experiment of U2OS using CL488-84056-6

    FC experiment of U2OS using CL488-84056-6

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Casq2 Recombinant Antibody (CL488-84056-6, Clone:241211A3)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of A549 using CL488-84056-6

    FC experiment of A549 using CL488-84056-6

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Coralite® Plus 488 Casq2 Recombinant Antibody (CL488-84056-6, Clone:241211A3)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control Recombinant Antibody (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inC2C12 cells
Positive FC (Intra) detected inU2OS cells, A549 cells
For other applications, we recommend the unconjugated version of this antibody, 84056-6-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84056-6 targets CASQ2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CASQ2 fusion protein Ag33584 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calsequestrin 2 (cardiac muscle)
Synonyms Calsequestrin-2, Calsequestrin 2, 241211A3
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46-55 kDa
GenBank Accession NumberBC022288
Gene Symbol Calsequestrin 2
Gene ID (NCBI) 845
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14958
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CASQ2 antibody CL488-84056-6Download protocol
Standard Protocols
Click here to view our Standard Protocols