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Calpastatin Monoclonal antibody

Calpastatin Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G12A2

Cat no : 67107-1-Ig

Print datasheet

Synonyms

CAST, Sperm BS-17 component, Calpain inhibitor, 1G12A2

验证数据展示

  • WB analysis of HeLa using 67107-1-Ig

    WB analysis of HeLa using 67107-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (CAST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67107-1-Ig

    IF Staining of HeLa using 67107-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpastatin antibody (67107-1-Ig, Clone: 1G12A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 67107-1-Ig

    FC experiment of HeLa using 67107-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Calpastatin Monoclonal antibody (67107-1-Ig, Clone:1G12A2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, MCF-7 cells, THP-1 cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67107-1-Ig targets Calpastatin in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen Calpastatin fusion protein Ag28669 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calpastatin
Synonyms CAST, Sperm BS-17 component, Calpain inhibitor, 1G12A2
Calculated Molecular Weight 667 aa, 72 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC013579
Gene Symbol Calpastatin
Gene ID (NCBI) 831
RRIDAB_2882411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20810
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Calpastatin antibody 67107-1-IgDownload protocol
IF protocol for Calpastatin antibody 67107-1-IgDownload protocol
FC protocol for Calpastatin antibody 67107-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Oxid Med Cell Longev

Polysaccharides from Polygonatum cyrtonema Hua Reduce Depression-Like Behavior in Mice by Inhibiting Oxidative Stress-Calpain-1-NLRP3 Signaling Axis.

Authors - Fengming Shen
View Article
mouseWB

Pharmacol Res

Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

Authors - Juan Wang
View Article