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CoraLite®594-conjugated Calpastatin Monoclonal antibody

Calpastatin Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G12A2

Cat no : CL594-67107

Print datasheet

Synonyms

BS 17, Calpain inhibitor, calpastatin, CAST, Sperm BS 17 component

验证数据展示

  • IF Staining of HeLa using CL594-67107

    IF Staining of HeLa using CL594-67107

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Calpastatin antibody (CL594-67107, Clone: 1G12A2 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67107

    FC experiment of HeLa using CL594-67107

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Calpastatin (CL594-67107, Clone:1G12A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67107-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67107 targets Calpastatin in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human
Immunogen Calpastatin fusion protein Ag28669 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calpastatin
Synonyms BS 17, Calpain inhibitor, calpastatin, CAST, Sperm BS 17 component
Calculated Molecular Weight 667 aa, 72 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC013579
Gene Symbol Calpastatin
Gene ID (NCBI) 831
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20810
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 Calpastatin antibody CL594-67107Download protocol
FC protocol for CL594 Calpastatin antibody CL594-67107Download protocol
Standard Protocols
Click here to view our Standard Protocols