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验证数据展示

  • IF Staining of HeLa using CL594-67107

    IF Staining of HeLa using CL594-67107

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Calpastatin antibody (CL594-67107, Clone: 1G12A2 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67107

    FC experiment of HeLa using CL594-67107

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Calpastatin (CL594-67107, Clone:1G12A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Calpastatin Monoclonal antibody

Calpastatin Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-67107

产品说明书

CloneNo. 1G12A2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

IF/ICC, FC (Intra)

别名

BS 17, Calpain inhibitor, calpastatin, CAST, Sperm BS 17 component

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67107-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67107 targets Calpastatin in IF/ICC, FC (Intra) applications and shows reactivity with Human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human
免疫原 Calpastatin fusion protein Ag28669 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 calpastatin
别名 BS 17, Calpain inhibitor, calpastatin, CAST, Sperm BS 17 component
计算分子量 667 aa, 72 kDa
观测分子量 115 kDa
GenBank蛋白编号BC013579
基因名称 Calpastatin
Gene ID (NCBI) 831
RRIDAB_3084747
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20810
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 Calpastatin antibody CL594-67107Download protocol
FC protocol for CL594 Calpastatin antibody CL594-67107Download protocol
Standard Protocols
Click here to view our Standard Protocols