• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68244-1-Ig

    WB analysis using 68244-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68244-1-Ig (CBX2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HEK-293 using 68244-1-Ig

    IF Staining of HEK-293 using 68244-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CBX2 antibody (68244-1-Ig, Clone: 1D3H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CBX2 Monoclonal antibody

CBX2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68244-1-Ig

产品说明书

CloneNo. 1D3H3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

SRXY5, M33, CDCA6, 1D3H3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68244-1-Ig targets CBX2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CBX2 fusion protein Ag8311 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 chromobox homolog 2 (Pc class homolog, Drosophila)
别名 SRXY5, M33, CDCA6, 1D3H3
计算分子量 56 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC004252
基因名称 CBX2
Gene ID (NCBI) 84733
RRIDAB_2935331
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ14781
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The chromobox homolog protein 2 (CBX2) is a critical component of the PRC1 complex involved in antiviral innate immunity, neuronal, and gonadal differentiation, axial patterning during embryonic development, cell proliferation, and senescence (PMID: 32870972). CBX2 has 2 isoforms with the molecular mass of 23 and 56 kDa. Sometimes higher molecular weight around 65-70 kDa can also be observed, which may be a modified variant of CBX2.

实验方案

Product Specific Protocols
WB protocol for CBX2 antibody 68244-1-IgDownload protocol
IF protocol for CBX2 antibody 68244-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols