验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 68244-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68244 targets CBX2 in FC (Intra) applications and shows reactivity with Human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Human |
| 免疫原 | CBX2 fusion protein Ag8311 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | chromobox homolog 2 (Pc class homolog, Drosophila) |
| 别名 | SRXY5, M33, CDCA6, 1D3H3 |
| 计算分子量 | 56 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC004252 |
| 基因名称 | CBX2 |
| Gene ID (NCBI) | 84733 |
| RRID | AB_3084454 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q14781 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The chromobox homolog protein 2 (CBX2) is a critical component of the PRC1 complex involved in antiviral innate immunity, neuronal, and gonadal differentiation, axial patterning during embryonic development, cell proliferation, and senescence (PMID: 32870972). CBX2 has 2 isoforms with the molecular mass of 23 and 56 kDa. Sometimes higher molecular weight around 65-70 kDa can also be observed, which may be a modified variant of CBX2.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CBX2 antibody CL488-68244 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |