CoraLite® Plus 488-conjugated CCAR1 Recombinant antibody

CCAR1 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83936-4
CloneNo. 240980C12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -


经过测试的应用

Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CCAR1 Ready-To-Use IHC Kit. CCAR1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83936-4-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83936-4 targets CCAR1 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CCAR1 fusion protein Ag18786 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cell division cycle and apoptosis regulator 1
Synonyms
Calculated Molecular Weight 1150 aa, 133 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC132725
Gene Symbol CCAR1
Gene ID (NCBI) 55749
RRIDAB_3673327
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IX12
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cell cycle and apoptosis regulator 1 (CCAR1) was originally identified from a functional screening and implicated in retinoid-induced cell cycle arrest and apoptosis. It has been shown that, in breast cancer cells, CCAR1 regulates apoptosis induced by various agents, including chemotherapeutics adriamycin, etoposide, and EGFR inhibitor. It is also involved in mediating anchorage-independent growth of colon carcinoma cells. In addition, CCAR1 plays the role as a co-activator of nuclear receptors to recruit mediator complex. It was found to associate with components of the Mediator and p160 coactivator complexes and is recruited to endogenous NR target genes. CCAR1 is also a binding partner of b-catenin and assists b-catenin in transcriptional activation of Wnt target genes.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CCAR1 antibody CL488-83936-4Download protocol
Standard Protocols
Click here to view our Standard Protocols
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