验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
For other applications, we recommend the unconjugated version of this antibody, 82998-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82998-4 targets CCDC23 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | CCDC23 fusion protein Ag33650 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | coiled-coil domain containing 23 |
| 别名 | SVBP, Small vasohibin-binding protein, Coiled coil domain-containing protein 23, 230175B2 |
| GenBank蛋白编号 | BC029427 |
| 基因名称 | CCDC23 |
| Gene ID (NCBI) | 374969 |
| RRID | AB_3673167 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q8N300 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCDC23 also known as SVBP is a small vasohibin-binding protein of 66 amino acids, and functions as a chaperone of VASH1 in vascular endothelial cells(PMID: 20736312). SVBP enhances the tyrosine carboxypeptidase activity of VASH1 and VASH2, thereby promoting the removal of the C-terminal tyrosine residue of alpha-tubulin(PMID: 29146869, 31171830). Mutations in SVBP cause Neurodevelopmental disorders with ataxia, hypotonia, and microcephaly (NEDAHM)(PMID: 30607023, 31363758).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CCDC23 antibody CL488-82998-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |