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CCDC50 Monoclonal antibody, PBS Only

CCDC50 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

2F12G7

Cat no : 68369-1-PBS

Print datasheet

Synonyms

YMER, Protein Ymer, DFNA44, Coiled-coil domain-containing protein 50, C3orf6

验证数据展示

  • FC experiment of A549 using 68369-1-Ig (same clone as 68369-1-PBS)

    FC experiment of A549 using 68369-1-Ig (same clone as 68369-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug CCDC50 Monoclonal antibody (68369-1-Ig, Clone:2F12G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.

  • WB analysis using 68369-1-Ig (same clone as 68369-1-PBS)

    WB analysis using 68369-1-Ig (same clone as 68369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68369-1-Ig (CCDC50 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.

产品信息

68369-1-PBS targets CCDC50 in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity human
Immunogen CCDC50 fusion protein Ag15889 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name coiled-coil domain containing 50
Synonyms YMER, Protein Ymer, DFNA44, Coiled-coil domain-containing protein 50, C3orf6
Calculated Molecular Weight 482 aa, 56 kDa
Observed Molecular Weight 36-38 kDa
GenBank Accession NumberBC065004
Gene Symbol CCDC50
Gene ID (NCBI) 152137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IVM0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)