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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of A549 using 68369-1-Ig (same clone as 68369-1-PBS)

    FC experiment of A549 using 68369-1-Ig (same clone as 68369-1-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.4 ug CCDC50 Monoclonal antibody (68369-1-Ig, Clone:2F12G7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.

  • WB analysis using 68369-1-Ig (same clone as 68369-1-PBS)

    WB analysis using 68369-1-Ig (same clone as 68369-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68369-1-Ig (CCDC50 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68369-1-PBS in a different storage buffer formulation.

CCDC50 Monoclonal antibody, PBS Only

CCDC50 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68369-1-PBS

产品说明书

CloneNo. 2F12G7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Indirect ELISA, WB

别名

YMER, Protein Ymer, DFNA44, Coiled-coil domain-containing protein 50, C3orf6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68369-1-PBS targets CCDC50 in Indirect ELISA, WB applications and shows reactivity with human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 human
免疫原 CCDC50 fusion protein Ag15889 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coiled-coil domain containing 50
别名 YMER, Protein Ymer, DFNA44, Coiled-coil domain-containing protein 50, C3orf6
计算分子量 482 aa, 56 kDa
观测分子量 36-38 kDa
GenBank蛋白编号BC065004
基因名称 CCDC50
Gene ID (NCBI) 152137
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8IVM0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CCDC50 encodes Ymer, an effector of epidermal growth factor (EGF)-mediated cell signaling that is ubiquitously expressed in different organs and has been suggested to inhibit down-regulation of the EGF receptor. CCDC50 act as a multifunctional regulator in NF-κB and Fas signaling pathways. In chronic lymphocytic leukaemia (CLL) and mantle cell lymphoma (MCL), CCDC50 is required for the survival of MCL and CLL cancer cells. Recently, CCDC50 was shown to promote hepatocellular carcinoma through the Ras/Foxo4 signaling pathway. (PMID: 34453126, PMID: 32612200, PMID: 17503326)