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验证数据展示

  • WB analysis using 68341-1-Ig

    WB analysis using 68341-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68341-1-Ig (CCS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 68341-1-Ig

    WB analysis of HepG2 using 68341-1-Ig

    WB result of CCS antibody (68341-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCS transfected HepG2 cells.

  • IF Staining of HepG2 using 68341-1-Ig

    IF Staining of HepG2 using 68341-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CCS antibody (68341-1-Ig, Clone: 3F5E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of mouse brain using 68341-1-Ig

    IHC staining of mouse brain using 68341-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68341-1-Ig (CCS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CCS Monoclonal antibody

CCS Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68341-1-Ig

产品说明书

CloneNo. 3F5E5

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CCS

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HepG2 cells, A549 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68341-1-Ig targets CCS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 CCS fusion protein Ag18860 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 copper chaperone for superoxide dismutase
别名 CCS
计算分子量 274 aa, 29 kDa
观测分子量32 kDa
GenBank蛋白编号BC105016
基因名称 CCS
Gene ID (NCBI) 9973
RRIDAB_3085065
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14618
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.

实验方案

Product Specific Protocols
WB protocol for CCS antibody 68341-1-IgDownload protocol
IHC protocol for CCS antibody 68341-1-IgDownload protocol
IF protocol for CCS antibody 68341-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols