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验证数据展示

  • IF Staining of HepG2 using CL488-68341

    IF Staining of HepG2 using CL488-68341

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 CCS antibody (CL488-68341, Clone: 3F5E5 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CCS Monoclonal antibody

CCS Monoclonal Antibody for IF/ICC
Cat No. CL488-68341

Print datasheet

CloneNo. 3F5E5

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

CCS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68341-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68341 targets CCS in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen CCS fusion protein Ag18860 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name copper chaperone for superoxide dismutase
Synonyms CCS
Calculated Molecular Weight 274 aa, 29 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC105016
Gene Symbol CCS
Gene ID (NCBI) 9973
RRIDAB_3084476
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14618
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Copper chaperone for superoxide dismutase (CCS) is a soluble factor that converts copper into copper-zinc superoxide dismutase ( SOD1 ). CCS and SOD1 interact directly in vivo and in vitro, and this interaction is mediated by homologous domains in each protein. CCS not only interacts with wild-type SOD1 but also interacts with SOD1 containing common missense mutations, resulting in FALS.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CCS antibody CL488-68341Download protocol
Standard Protocols
Click here to view our Standard Protocols