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验证数据展示

  • WB analysis using 84658-5-RR

    WB analysis using 84658-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84658-5-RR (CD107a / LAMP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 84658-5-RR

    WB analysis of HEK-293 using 84658-5-RR

    WB result of CD107a / LAMP1 antibody (84658-5-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD107a / LAMP1 transfected HEK-293 cells.

  • IF Staining of HeLa using 84658-5-RR

    IF Staining of HeLa using 84658-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD107a / LAMP1 antibody (84658-5-RR, Clone: 242116E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of human liver cancer using 84658-5-RR

    IHC staining of human liver cancer using 84658-5-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84658-5-RR (CD107a / LAMP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LAMP1/CD107a antibody included
  • IHC staining of human liver cancer using 84658-5-RR

    IHC staining of human liver cancer using 84658-5-RR

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84658-5-RR (CD107a / LAMP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LAMP1/CD107a antibody included
  • Featured Product
  • KD/KO验证

CD107a / LAMP1 Recombinant monoclonal antibody

CD107a / LAMP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84658-5-RR

产品说明书

CloneNo. 242116E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD107a, LAMP1, 242116E8, CD107 antigen-like family member A, CD107a/LAMP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84658-5-RR targets CD107a / LAMP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2611

Product name: Recombinant Human CD107a/LAMP1 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 26-381 aa of NM_005561.4

Sequence: ASAAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lysosomal-associated membrane protein 1
别名 CD107a, LAMP1, 242116E8, CD107 antigen-like family member A, CD107a/LAMP1
计算分子量45 kDa
观测分子量100-120 kDa
GenBank蛋白编号NM_005561.4
基因名称 LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP11279-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

实验方案

Product Specific Protocols
IF protocol for CD107a / LAMP1 antibody 84658-5-RRDownload protocol
IHC protocol for CD107a / LAMP1 antibody 84658-5-RRDownload protocol
WB protocol for CD107a / LAMP1 antibody 84658-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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