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验证数据展示

  • FC experiment of human PBMCs using CL647-65520

    FC experiment of human PBMCs using CL647-65520

    1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b Rabbit Recombinant Antibody (CL647-65520, Clone:H4B4) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

  • FC experiment of human PBMCs using CL647-65520

    FC experiment of human PBMCs using CL647-65520

    1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b Rabbit Recombinant Antibody (CL647-65520, Clone:H4B4) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

CoraLite® Plus 647 Anti-Human CD107b / LAMP2 (H4B4) Rabbit IgG Recombinant Antibody

CD107b / LAMP2 Recombinant Antibody for FC (Intra)
Cat No. CL647-65520

产品说明书

CloneNo. H4B4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

CD107b, LAMP2, LAMP-2, LAMP 2, H4B4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65520-1-PBS

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65520 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Human adherent peripheral blood cells 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lysosomal-associated membrane protein 2
别名 CD107b, LAMP2, LAMP-2, LAMP 2, H4B4
计算分子量 45 kDa
GenBank蛋白编号BC002965
基因名称 LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
RRIDAB_3673596
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD107b / LAMP2 antibody CL647-65520Download protocol
Standard Protocols
Click here to view our Standard Protocols