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验证数据展示

  • FC experiment of human PBMCs using FITC-65520

    FC experiment of human PBMCs using FITC-65520

    1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD107b Rabbit Recombinant Antibody (FITC-65520, Clone:H4B4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

  • FC experiment of human PBMCs using FITC-65520

    FC experiment of human PBMCs using FITC-65520

    1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD107b Rabbit Recombinant Antibody (FITC-65520, Clone:H4B4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

FITC Plus Anti-Human CD107b / LAMP2 (H4B4) Rabbit IgG Recombinant Antibody

CD107b / LAMP2 Recombinant Antibody for FC (Intra)
Cat No. FITC-65520
CloneNo. H4B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

CD107b, LAMP2, LAMP-2, LAMP 2, H4B4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  FITC Plus
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65520 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Human adherent peripheral blood cells 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lysosomal-associated membrane protein 2
Synonyms CD107b, LAMP2, LAMP-2, LAMP 2, H4B4
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
RRIDAB_3673860
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD107b / LAMP2 antibody FITC-65520Download protocol
Standard Protocols
Click here to view our Standard Protocols