验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Jurkat cells |
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-65566 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | Human adherent peripheral blood cells 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Full Name | lysosomal-associated membrane protein 2 |
| Synonyms | CD107b, LAMP2, LAMP-2, LAMP 2, H4B4 |
| Calculated Molecular Weight | 45 kDa |
| GenBank Accession Number | BC002965 |
| Gene Symbol | LAMP2 |
| Gene ID (NCBI) | 3920 |
| ENSEMBL Gene ID | ENSG00000005893 |
| Conjugate | Biotin |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin CD107b / LAMP2 antibody Biotin-65566 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |