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CoraLite® Plus 647 Anti-Human CD107b / LAMP2 (H4B4) Mouse IgG2a Recombinant Antibody

CD107b / LAMP2 Recombinant Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

H4B4

Cat No : CL647-65566

Print datasheet

Synonyms

CD107b, LAMP 2, LAMP2, LAMPB, LGP110

验证数据展示

  • FC experiment of Jurkat using CL647-65566

    FC experiment of Jurkat using CL647-65566

    1x10^6 Jurkat cells were intracellularly stained with 5 ul Coralite® Plus 647 CD107b / LAMP2 Recombinant Antibody (CL647-65566, Clone:H4B4) (red) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65566-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65566 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen Human adherent peripheral blood cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name lysosomal-associated membrane protein 2
Synonyms CD107b, LAMP 2, LAMP2, LAMPB, LGP110
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD107b / LAMP2 antibody CL647-65566Download protocol
Standard Protocols
Click here to view our Standard Protocols