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PE Anti-Human CD117/c-Kit (104D2)

CD117/c-Kit Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Bovine

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

104D2

Cat no : PE-65154

Print datasheet

Synonyms

C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit

验证数据展示

  • FC experiment of TF-1 using PE-65154

    FC experiment of TF-1 using PE-65154

    1X10^6 TF-1 cells were surface stained with 20.00 ul/test PE Anti-Human CD117 (PE-65154, Clone:104D2) (red) or isotype control antibody (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inTF-1 cells
Planning an IHC experiment? We recommend our IHCeasy CD117/c-kit Ready-To-Use IHC Kit. CD117/c-kit primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65154 targets CD117/c-Kit in FC applications and shows reactivity with Human, Bovine samples.

Tested Applications FC Application Description
Tested Reactivity Human, Bovine
Immunogen MOLM-1 megakaryocytic cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
Synonyms C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit
Calculated Molecular Weight 976 aa, 110 kDa
GenBank Accession NumberBC071593
Gene Symbol CD117
Gene ID (NCBI) 3815
RRIDAB_2883933
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDP10721
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD117, also known as c-Kit and SCFR, is a transmembrane protein with tyrosine kinase activity encoded by the oncogene c-kit (PMID: 2448137). It is a member of the type III receptor tyrosine kinase family, which also includes CSF-1R, PDGFRβ, PDGFRα, and FLT3 (PMID: 29518044). CD117 is expressed on hematopoietic stem cells and progenitor cells, mast cells, and is also found in a wide range of non-haemopoietic cell types (including melanocytes, germ cells, astrocytes, renal tubules, breast glandular epithelial cells, sweat glands, and interstitial cells of Cajal) (PMID: 10582338; 23073628). CD117 plays an important role in early haemopoiesis. It is also involved in pigmentation, fertility, gut movement, and some aspects of the nervous system (PMID: 23073628).

实验方案

Product Specific Protocols
FC protocol for PE CD117/c-Kit antibody PE-65154Download protocol
Standard Protocols
Click here to view our Standard Protocols