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PerCP-Cyanine5.5 Anti-Human CD11a (TS2/4) Mouse IgG2a Recombinant Antibody

CD11a Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

TS2/4

Cat No : CPY5-65563

Print datasheet

Synonyms

Leukocyte function-associated molecule 1 alpha chain, Leukocyte adhesion glycoprotein LFA-1 alpha chain, ITGAL, Integrin alpha-L, Integrin alpha L

验证数据展示

  • FC experiment of human PBMCs using CPY5-65563

    FC experiment of human PBMCs using CPY5-65563

    1x10^6 human PBMCs were surface stained with 5 ul PerCP-Cyanine5.5 Anti-Human CD11a (TS2/4) Mouse IgG2a Recombinant Antibody (CPY5-65563, Clone:TS2/4) or unstained. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CPY5-65563 targets CD11a in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen HLA-DR CTL line 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
Synonyms Leukocyte function-associated molecule 1 alpha chain, Leukocyte adhesion glycoprotein LFA-1 alpha chain, ITGAL, Integrin alpha-L, Integrin alpha L
Calculated Molecular Weight 129 kDa
GenBank Accession NumberBC008777
Gene Symbol CD11a
Gene ID (NCBI) 3683
ENSEMBL Gene IDENSG00000005844
Conjugate PerCP-Cyanine5.5 Fluorescent Dye
Excitation/Emission Maxima Wavelengths482 nm / 677 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20701
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD11a, also known as integrin αL or LFA-1α, is a 170-180 kDa transmembrane glycoprotein that forms a heterodimer with CD18 (PMID: 7027264; 1672643). CD11a/CD18 (LFA-1) is expressed by all leukocytes and mediates cell adhesion through interactions with its ligands, intercellular adhesion molecule 1 (ICAM-1), ICAM-2, and ICAM-3 (PMID: 7479767). CD11a/CD18 also functions in lymphocyte costimulatory signaling (PMID: 1972160).

实验方案

Product Specific Protocols
FC protocol for PerCP-Cyanine5.5 CD11a antibody CPY5-65563Download protocol
Standard Protocols
Click here to view our Standard Protocols