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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of mouse bone marrow cells using CL555-65055

    FC experiment of mouse bone marrow cells using CL555-65055

    1X10^6 mouse bone marrow cells were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD11b (CL555-65055, Clone: M1/70) (red), or 0.5 ug Isotype Control. Cells were not fixed.

  • FC experiment of mouse bone marrow cells using CL555-65055

    FC experiment of mouse bone marrow cells using CL555-65055

    1X10^6 mouse bone marrow cells were surface stained with 0.5 ug CoraLite® Plus 555 Anti-Mouse CD11b (CL555-65055, Clone: M1/70), or 0.5 ug Isotype Control. Cells were not fixed.

CoraLite® Plus 555 Anti-Mouse CD11b (M1/70)

CD11b Monoclonal Antibody for FC
Cat No. CL555-65055
CloneNo. M1/70

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Synonyms

M1/70, Ly 40, Itgam, integrin alpha M, CR3A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 555
Size: 

-/ -

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经过测试的应用

Positive FC detected inmouse bone marrow cells
For other applications, we recommend the unconjugated version of this antibody, 65055-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65055 targets CD11b in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name integrin alpha M
Synonyms M1/70, Ly 40, Itgam, integrin alpha M, CR3A
Calculated Molecular Weight127 kDa
GenBank Accession NumberBC156991
Gene Symbol CD11b
Gene ID (NCBI) 16409
RRIDAB_3084538
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDE9Q604
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 CD11b antibody CL555-65055Download protocol
Standard Protocols
Click here to view our Standard Protocols