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CoraLite® Plus 647 Anti-Human CD11c (BU15) Mouse IgG2a Recombinant Antibody

CD11c Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

BU15

Cat no : CL647-65600

Print datasheet

Synonyms

Leu M5, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

验证数据展示

  • FC experiment of human peripheral blood leukocytes using CL647-65600

    FC experiment of human peripheral blood leukocytes using CL647-65600

    1x10^6 human peripheral blood leukocytes were surface stained with CoraLite® Plus 488 Anti-Human CD14 and 5 ul CoraLite® Plus 647 Anti-Human CD11c Mouse IgG2a Recombinant Antibody (CL647-65600, Clone: BU15), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (CL647-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using CL647-65600

    FC experiment of human peripheral blood leukocytes using CL647-65600

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD11c Mouse IgG2a Recombinant Antibody (CL647-65600, Clone: BU15) (red), or CoraLite® Plus 647 Mouse IgG2a Isotype Control (CL647-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65600-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65600 targets CD11c in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human synovial fluid dendritic cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Synonyms Leu M5, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C
Calculated Molecular Weight 1169 aa, 129 kDa
GenBank Accession NumberBC038237
Gene Symbol CD11c
Gene ID (NCBI) 3687
ENSEMBL Gene IDENSG00000140678
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts as a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD11c antibody CL647-65600Download protocol
Standard Protocols
Click here to view our Standard Protocols