验证数据展示
经过测试的应用
| Positive IF-P detected in | human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy CD11c Ready-To-Use IHC Kit. CD11c primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60258-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 3 publications below |
产品信息
CL488-60258 targets CD11c/Integrin Alpha X in IF-P applications and shows reactivity with human samples.
| Tested Applications | IF-P Application Description |
| Cited Applications | IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | CD11c/Integrin Alpha X fusion protein Ag11350 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | integrin, alpha X (complement component 3 receptor 4 subunit) |
| Synonyms | CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6 |
| Calculated Molecular Weight | 1169 aa, 129 kDa |
| GenBank Accession Number | BC038237 |
| Gene Symbol | CD11c |
| Gene ID (NCBI) | 3687 |
| ENSEMBL Gene ID | ENSG00000140678 |
| RRID | AB_2883128 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20702 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis. And this anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD11c/Integrin Alpha X antibody CL488-60258 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Cancer Res 18F-FMISO PET Imaging Identifies Hypoxia and Immunosuppressive Tumor Microenvironments and Guides Targeted Evofosfamide Therapy in Tumors Refractory to PD-1 and CTLA-4 Inhibition. | ||
Respir Res TFEB regulates dendritic cell antigen presentation to modulate immune balance in asthma | ||
Inflamm Res Long-chain acyl-CoA synthetase 4-mediated mitochondrial fatty acid metabolism and dendritic cell antigen presentation |