CoraLite® Plus 488 Anti-Human IL3RA/CD123 (HI12H7)
IL3RA/CD123 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
human
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
HI12H7
验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 65256-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65256 targets IL3RA/CD123 in FC applications and shows reactivity with human samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | human |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interleukin 3 receptor, alpha (low affinity) |
| Synonyms | IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra |
| Calculated Molecular Weight | 378 aa, 43 kDa |
| GenBank Accession Number | BC035407 |
| Gene Symbol | CD123 |
| Gene ID (NCBI) | 3563 |
| ENSEMBL Gene ID | ENSG00000185291 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD123, also referred to as interleukin-3 receptor subunit alpha (IL-3Rα), belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). CD123 is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to CD123 and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 IL3RA/CD123 antibody CL488-65256 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
