验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-65256 targets IL3RA/CD123 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 | N/A 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interleukin 3 receptor, alpha (low affinity) |
| 别名 | IL3RA, IL 3R subunit alpha, IL 3R alpha, IL 3 receptor subunit alpha, hIL 3Ra |
| 计算分子量 | 378 aa, 43 kDa |
| GenBank蛋白编号 | BC035407 |
| 基因名称 | CD123 |
| Gene ID (NCBI) | 3563 |
| ENSEMBL Gene ID | ENSG00000185291 |
| RRID | AB_3085026 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD123, also referred to as interleukin-3 receptor subunit alpha (IL-3Rα), belongs to the cytokine receptor family. It is a glycoprotein containing an extracellular domain, involving a predicted Ig-like domain, two FnIII domains, a transmembrane domain and an intracellular domain (PMID: 24513123). CD123 is expressed on hematopoietic progenitor cells, plasmacytoid dendritic cells, basophils, monocytes, some B cells, and in a variety of hematologic malignancies (PMID: 10527461; 34855461). IL-3 initially binds to CD123 and subsequently recruits the beta chain (CD131) to form the high-affinity receptor, resulting in the activation of a series of signaling pathways including the JAK/STAT, Ras-MAPK, and phosphatidylinositol 3-kinase pathways (PMID: 29296749). IL-3R signaling regulates the proliferation, survival, and differentiation of hematopoietic cells.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE IL3RA/CD123 antibody PE-65256 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |