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Cardinal Red™ Anti-Human CD13 (WM15)

CD13 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

Cardinal Red™ Fluorescent Dye

CloneNo.

WM15

Cat no : CR-65186

Print datasheet

Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN

验证数据展示

  • FC experiment of human PBMCs using CR-65186

    FC experiment of human PBMCs using CR-65186

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD13 (CR-65186, Clone:WM15) (red) or unstained. Cells were not fixed. Granulocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD13 Ready-To-Use IHC Kit. CD13 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-65186 targets CD13 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Applications FC Application Description
Tested Reactivity Human, Non-human primates
Immunogen Human AML cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name alanyl (membrane) aminopeptidase
Synonyms Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN
Calculated Molecular Weight 110 kDa
GenBank Accession NumberBC058928
Gene Symbol CD13
Gene ID (NCBI) 290
ENSEMBL Gene IDENSG00000166825
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths592 nm / 611 nm
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP15144
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation, and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

实验方案

Product Specific Protocols
FC protocol for Cardinal Red™ CD13 antibody CR-65186Download protocol
Standard Protocols
Click here to view our Standard Protocols