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Anti-Human CD13 (WM15)

CD13 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human, non-human primates

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

WM15

Cat no : 65186-1-Ig

Print datasheet

Synonyms

APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M

验证数据展示

  • IF Staining of HUVEC using 65186-1-Ig

    IF Staining of HUVEC using 65186-1-Ig

    Immunofluorescent analysis of un-fixed HUVEC cells using CD13 antibody (65186-1-Ig, Clone: WM15 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • FC experiment of human PBMCs using 65186-1-Ig

    FC experiment of human PBMCs using 65186-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using 65186-1-Ig

    FC experiment of human PBMCs using 65186-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD13 (65186-1-Ig, Clone:WM15) and FITC-Goat anti-Mouse IgG at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

  • IF Staining of THP-1 using 65186-1-Ig

    IF Staining of THP-1 using 65186-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD13 antibody (65186-1-Ig, Clone: WM15 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive IF/ICC detected inHUVEC cells, THP-1 cells
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD13 Ready-To-Use IHC Kit. CD13 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 555, CoraLite® Plus 647 and CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65186-1-Ig targets CD13 in IF/ICC, FC applications and shows reactivity with human, non-human primates samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity human, non-human primates
Immunogen Human AML cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name alanyl (membrane) aminopeptidase
Synonyms APN, AP-M, ANPEP, Aminopeptidase N, Aminopeptidase M
Calculated Molecular Weight 110 kDa
GenBank Accession NumberBC058928
Gene Symbol CD13
Gene ID (NCBI) 290
ENSEMBL Gene IDENSG00000166825
RRIDAB_2918457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15144
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)

实验方案

Product Specific Protocols
IF protocol for CD13 antibody 65186-1-IgDownload protocol
FC protocol for CD13 antibody 65186-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols