• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using APC-65246

    FC experiment of human PBMCs using APC-65246

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC) or APC Mouse IgG2b Isotype Control (MPC-11) (APC-65128, Clone: MPC-11). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using APC-65246

    FC experiment of human PBMCs using APC-65246

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC) (red) or APC Mouse IgG2b Isotype Control (MPC-11) (APC-65128, Clone: MPC-11) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

APC Anti-Human CD14 (26IC)

CD14 Monoclonal Antibody for FC
Cat No. APC-65246

Print datasheet

CloneNo. 26IC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC

Synonyms

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 26IC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  APC
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65246 targets CD14 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD14 molecule
Synonyms Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 26IC
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_2935217
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08571
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
FC protocol for APC CD14 antibody APC-65246Download protocol
Standard Protocols
Click here to view our Standard Protocols