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验证数据展示

  • FC experiment of human PBMCs using APC-65246

    FC experiment of human PBMCs using APC-65246

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC) or APC Mouse IgG2b Isotype Control (MPC-11) (APC-65128, Clone: MPC-11). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using APC-65246

    FC experiment of human PBMCs using APC-65246

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC) (red) or APC Mouse IgG2b Isotype Control (MPC-11) (APC-65128, Clone: MPC-11) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

APC Anti-Human CD14 (26IC)

CD14 Monoclonal Antibody for FC
Cat No. APC-65246

产品说明书

CloneNo. 26IC

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

FC

别名

Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 26IC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  APC
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65246 targets CD14 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD14 molecule
别名 Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule, 26IC
计算分子量 375 aa, 40 kDa
GenBank蛋白编号BC010507
基因名称 CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_2935217
偶联类型 APC Fluorescent Dye
最大激发/发射波长650 nm / 660 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP08571
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
FC protocol for APC CD14 antibody APC-65246Download protocol
Standard Protocols
Click here to view our Standard Protocols