CoraLite® Plus 647 Anti-Human CD14 (26IC)
CD14 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
26IC
验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65246-1-Ig
推荐稀释比
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-65246 targets CD14 in FC applications and shows reactivity with Human samples.
Tested Applications | FC Application Description |
Tested Reactivity | Human |
Immunogen | N/A 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | CD14 molecule |
Synonyms | CD14, CD14 molecule |
Calculated Molecular Weight | 375 aa, 40 kDa |
GenBank Accession Number | BC010507 |
Gene Symbol | CD14 |
Gene ID (NCBI) | 929 |
ENSEMBL Gene ID | ENSG00000170458 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P08571 |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 CD14 antibody CL647-65246 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |