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验证数据展示

  • FC experiment of human PBMCs using CL647-65246

    FC experiment of human PBMCs using CL647-65246

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD14 (CL647-65246, Clone:26IC) (red) or unstained. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using CL647-65246

    FC experiment of human PBMCs using CL647-65246

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD14 (CL647-65246, Clone: 26IC) or unstained. Cells were not fixed. Monocytes were gated.

CoraLite® Plus 647 Anti-Human CD14 (26IC)

CD14 Monoclonal Antibody for FC
Cat No. CL647-65246

产品说明书

CloneNo. 26IC

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

FC

别名

CD14, CD14 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65246-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65246 targets CD14 in FC applications and shows reactivity with Human samples.

经测试应用 FC Application Description
经测试反应性 Human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD14 molecule
别名 CD14, CD14 molecule
计算分子量 375 aa, 40 kDa
GenBank蛋白编号BC010507
基因名称 CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_2935028
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP08571
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD14 antibody CL647-65246Download protocol
Standard Protocols
Click here to view our Standard Protocols