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CoraLite® Plus 594 Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

UCHM-1

Cat no : CL594-65056

Print datasheet

Synonyms

UCHM-1, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule

验证数据展示

  • FC experiment of human PBMCs using CL594-65056

    FC experiment of human PBMCs using CL594-65056

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD14 (CL594-65056, Clone:UCHM-1) or CoraLite®594 Mouse IgG2a Isotype Control (CL594-65208, Clone: C1.18.4). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using CL594-65056

    FC experiment of human PBMCs using CL594-65056

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD14 (CL594-65056, Clone:UCHM-1) or CoraLite®594 Mouse IgG2a Isotype Control (CL594-65208, Clone: C1.18.4). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65056-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65056 targets CD14 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Thymocytes and peripheral blood lymphocytes from a remission AML patient 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD14 molecule
Synonyms UCHM-1, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
Conjugate CoraLite® Plus 594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths594 nm / 615 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08571
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
FC protocol for CL Plus 594 CD14 antibody CL594-65056Download protocol
Standard Protocols
Click here to view our Standard Protocols