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验证数据展示

  • FC experiment of human PBMCs using 65056-1-Ig

    FC experiment of human PBMCs using 65056-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD14 (65056-1-Ig, Clone: UCHM-1) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained (blue). Cells were not fixed. Monocytes were gated.

Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for FC
Cat No. 65056-1-Ig

Print datasheet

CloneNo. UCHM-1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

UCHM-1, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 
100 μg

¥780/ 100 μg

浓度: 0.5 mg/ml

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65056-1-Ig targets CD14 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Thymocytes and peripheral blood lymphocytes from a remission AML patient 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD14 molecule
Synonyms UCHM-1, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule
Calculated Molecular Weight 375 aa, 40 kDa
GenBank Accession NumberBC010507
Gene Symbol CD14
Gene ID (NCBI) 929
ENSEMBL Gene IDENSG00000170458
RRIDAB_2918364
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP08571
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
FC protocol for CD14 antibody 65056-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols