验证数据展示
经过测试的应用
| Positive FC detected in | C57BL/6 mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 4 publications below |
| FC | See 5 publications below |
产品信息
65057-1-Ig targets CD16/32 in IF, FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 文献引用应用 | IF, FC |
| 经测试反应性 | mouse |
| 文献引用反应性 | mouse |
| 免疫原 | VH81X Tg B cells and T220 stromal cell line 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2a, lambda |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Fc receptor, IgG, low affinity IIb |
| 别名 | CD32, 93, Fcr 2, FcgRII, Fcgr2b |
| GenBank蛋白编号 | BC038070 |
| 基因名称 | CD16/32 |
| Gene ID (NCBI) | 14130 |
| RRID | AB_2918365 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P08101 |
| 储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors by preincubating the cells with the purified antibody at 1 ug per million cells in 100 uL volume for 5-10 minutes prior to immunostaining.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CD16/32 antibody 65057-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater Ultrasound Imaging of Tumor Vascular CD93 with MMRN2 Modified Microbubbles for Immune Microenvironment Prediction | ||
Front Microbiol Response to HIV-1 gp160-carrying recombinant virus HSV-1 and HIV-1 VLP combined vaccine in BALB/c mice | ||
Cell Signal OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization | ||
Exp Brain Res Silencing CXCL16 alleviate neuroinflammation and M1 microglial polarization in mouse brain hemorrhage model and BV2 cell model through PI3K/AKT pathway | ||
Bioact Mater Reprogramming exosomes for immunity-remodeled photodynamic therapy against non-small cell lung cancer | ||
J Nanobiotechnology Bioengineered stem cell membrane functionalized nanoparticles combine anti-inflammatory and antimicrobial properties for sepsis treatment |