Anti-Mouse CD16/32 (93)

CD16/32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, lambda

Reactivity

Mouse and More (2)

Applications

FC and More (1)

Conjugate

Unconjugated

CloneNo.

93

Cat No : 65057-1-Ig

Print datasheet

Synonyms

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1



经过测试的应用

Positive FC detected inBALB/c mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

产品信息

65057-1-Ig targets CD16/32 in IF, FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Cited Applications FC, IF
Tested Reactivity Mouse
Cited Reactivitymouse, monkey
Immunogen VH81X Tg B cells and T220 stromal cell line 种属同源性预测
Host / Isotype Rat / IgG2a, lambda
Class Monoclonal
Type Antibody
Full Name Fc receptor, IgG, low affinity IIb
Synonyms AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1
GenBank accession numberBC038070
Gene symbol Fcgr2b
UniprotP08101
Gene ID (NCBI) 14130
RRIDAB_2918365
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.

实验方案

Product Specific Protocols
FC protocol for CD16/32 antibody 65057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
monkeyFC

Front Microbiol

Response to HIV-1 gp160-carrying recombinant virus HSV-1 and HIV-1 VLP combined vaccine in BALB/c mice

Authors - Beibei Zhang
mouseIF

Cell Signal

OTUB1's role in promoting OSCC development by stabilizing RACK1 involves cell proliferation, migration, invasion, and tumor-associated macrophage M1 polarization

Authors - Yunyun Li
mouseIF,FC

J Nanobiotechnology

Bioengineered stem cell membrane functionalized nanoparticles combine anti-inflammatory and antimicrobial properties for sepsis treatment

Authors - Lu Lu
mouseFC

Stem Cells Dev

Deer antler reserve mesenchyme cell-conditioned medium reduces the destruction of periodontitis in mice

Authors - Hongbing Lin
mouseIF

BMC Neurosci

KDM4A, involved in the inflammatory and oxidative stress caused by traumatic brain injury-hemorrhagic shock, partly through the regulation of the microglia M1 polarization

Authors - Jimin Cai