Anti-Mouse CD16/32 (93)

CD16/32 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, lambda

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

Unconjugated

CloneNo.

93

Cat No : 65057-1-Ig

Print datasheet

Synonyms

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1



经过测试的应用

Positive FC detected inBALB/c mouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

发表文章中的应用

FCSee 1 publications below

产品信息

65057-1-Ig targets CD16/32 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Cited ApplicationsFC
Tested Reactivity Mouse
Cited Reactivitymouse
Immunogen VH81X Tg B cells and T220 stromal cell line 种属同源性预测
Host / Isotype Rat / IgG2a, lambda
Class Monoclonal
Type Antibody
Full Name Fc receptor, IgG, low affinity IIb
Synonyms AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1
GenBank accession numberBC038070
Gene symbol Fcgr2b
UniprotP08101
Gene ID (NCBI) 14130
RRIDAB_2918365
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.

实验方案

Product Specific Protocols
FC protocol for CD16/32 antibody 65057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

Stem Cells Dev

Deer antler reserve mesenchyme cell-conditioned medium reduces the destruction of periodontitis in mice

Authors - Hongbing Lin