CoraLite® Plus 750 Anti-Mouse CD16/32 (93)
CD16/32 Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2a, lambda
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
93
验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65057-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65057 targets CD16/32 in FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Mouse |
| Immunogen | VH81X Tg B cells and T220 stromal cell line 种属同源性预测 |
| Host / Isotype | Rat / IgG2a, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Fc receptor, IgG, low affinity IIb |
| Synonyms | AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1 |
| GenBank Accession Number | BC038070 |
| Gene Symbol | CD16/32 |
| Gene ID (NCBI) | 14130 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P08101 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Rat anti mouse CD16/32 antibody, clone 93 is specific to the common epitope of CD16 and CD32. It can be used for blocking non-specific binding of immunoglobulins to the Fc receptors.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 CD16/32 antibody CL750-65057 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |