验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65057-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65057 targets CD16/32 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 | VH81X Tg B cells and T220 stromal cell line 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2a, lambda |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Fc receptor, IgG, low affinity IIb |
| 别名 | CD32, 93, Fcr 2, FcgRII, Fcgr2b |
| GenBank蛋白编号 | BC038070 |
| 基因名称 | CD16/32 |
| Gene ID (NCBI) | 14130 |
| RRID | AB_3083820 |
| 偶联类型 | CoraLite® Plus 405 Fluorescent Dye |
| 最大激发/发射波长 | 399 nm / 422 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P08101 |
| 储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CD16/32 antibody CL405-65057 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |