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Anti-Human CD163 (GHI/61) Mouse IgG2a Recombinant Antibody

CD163 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/61

Cat no : 65561-1-MR

Print datasheet

Synonyms

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61

验证数据展示

  • FC experiment of human PBMCs using 65561-1-MR

    FC experiment of human PBMCs using 65561-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using 65561-1-MR

    FC experiment of human PBMCs using 65561-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65561-1-MR targets CD163 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Glycoprotein preparation from hairy cell leukemia spleen 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD163 molecule
Synonyms Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61
Calculated Molecular Weight 1156 aa, 125 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86VB7
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

实验方案

Product Specific Protocols
FC protocol for CD163 antibody 65561-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols