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Anti-Human CD163 (GHI/61) Mouse IgG2a Recombinant Antibody, PBS Only

CD163 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

GHI/61

Cat no : 65561-1-PBS

Print datasheet

Synonyms

Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61

验证数据展示

  • FC experiment of human PBMCs using 65561-1-MR (same clone as 65561-1-PBS)

    FC experiment of human PBMCs using 65561-1-MR (same clone as 65561-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD11b. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65561-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65561-1-MR (same clone as 65561-1-PBS)

    FC experiment of human PBMCs using 65561-1-MR (same clone as 65561-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD163 Mouse IgG2a Recombinant Antibody (65561-1-MR, Clone:GHI/61) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65561-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.

产品信息

65561-1-PBS targets CD163 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Glycoprotein preparation from hairy cell leukemia spleen 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD163 molecule
Synonyms Scavenger receptor cysteine-rich type 1 protein M130, MM130, M130, Hemoglobin scavenger receptor, GHI/61
Calculated Molecular Weight 1156 aa, 125 kDa
GenBank Accession NumberBC051281
Gene Symbol CD163
Gene ID (NCBI) 9332
ENSEMBL Gene IDENSG00000177575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86VB7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).