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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83285-4-RR (same clone as 83285-4-PBS)

    WB analysis using 83285-4-RR (same clone as 83285-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 83285-4-RR (same clone as 83285-4-PBS)

    IHC staining of mouse liver using 83285-4-RR (same clone as 83285-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 83285-4-RR (same clone as 83285-4-PBS)

    IHC staining of mouse liver using 83285-4-RR (same clone as 83285-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 83285-4-RR (same clone as 83285-4-PBS)

    IF Staining of mouse small intestine using 83285-4-RR (same clone as 83285-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • IF Staining of rat liver using 83285-4-RR (same clone as 83285-4-PBS)

    IF Staining of rat liver using 83285-4-RR (same clone as 83285-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83285-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83285-4-RR

    Affinity and Kinetic Characterization of 83285-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83285-4-RR against Mouse CD163 were performed. The affinity constant is 2.25 nM.

Mouse CD163 Recombinant antibody, PBS Only

CD163 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83285-4-PBS
CloneNo. 240233A3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

sCD163, M130, 240233A3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

83285-4-PBS targets CD163 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD163 antigen
Synonyms sCD163, M130, 240233A3
Calculated Molecular Weight121 kDa
Observed Molecular Weight160-170 kDa
GenBank Accession NumberNM_053094.2
Gene Symbol Cd163
Gene ID (NCBI) 93671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ2VLH6-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).