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CD163 Recombinant antibody

CD163 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240233A3

Cat No : 83285-4-RR

Print datasheet

Synonyms

sCD163, M130, 240233A3

验证数据展示

  • WB analysis using 83285-4-RR

    WB analysis using 83285-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 83285-4-RR

    IHC staining of mouse spleen using 83285-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 83285-4-RR

    IHC staining of mouse spleen using 83285-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83285-4-RR

    Affinity and Kinetic Characterization of 83285-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83285-4-RR against Mouse CD163 were performed. The affinity constant is 2.25 nM.

经过测试的应用

Positive WB detected inmouse liver tissue, mouse lung tissue, rat spleen tissue, rat liver tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83285-4-RR is CD163 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD163 antigen
Synonyms sCD163, M130, 240233A3
Calculated Molecular Weight121 kDa
Observed Molecular Weight160-170 kDa
GenBank Accession NumberNM_053094.2
Gene Symbol Cd163
Gene ID (NCBI) 93671
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ2VLH6-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

实验方案

Product Specific Protocols
WB protocol for CD163 antibody 83285-4-RRDownload protocol
IHC protocol for CD163 antibody 83285-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols