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验证数据展示

  • WB analysis using 83285-4-RR

    WB analysis using 83285-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83285-4-RR (CD163 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 83285-4-RR

    IHC staining of mouse liver using 83285-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 83285-4-RR

    IHC staining of mouse liver using 83285-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83285-4-RR (CD163 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 83285-4-RR

    IF Staining of mouse small intestine using 83285-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat liver using 83285-4-RR

    IF Staining of rat liver using 83285-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD163 antibody (83285-4-RR, Clone: 240233A3 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83285-4-RR

    Affinity and Kinetic Characterization of 83285-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83285-4-RR against Mouse CD163 were performed. The affinity constant is 2.25 nM.

CD163 Recombinant antibody

CD163 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83285-4-RR

产品说明书

CloneNo. 240233A3

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

sCD163, M130, 240233A3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, mouse lung tissue, rat spleen tissue, rat liver tissue
Positive IHC detected inmouse liver tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue, rat liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83285-4-RR targets CD163 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD163 antigen
别名 sCD163, M130, 240233A3
计算分子量121 kDa
观测分子量160-170 kDa
GenBank蛋白编号NM_053094.2
基因名称 Cd163
Gene ID (NCBI) 93671
RRIDAB_3670952
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ2VLH6-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

实验方案

Product Specific Protocols
WB protocol for CD163 antibody 83285-4-RRDownload protocol
IHC protocol for CD163 antibody 83285-4-RRDownload protocol
IF protocol for CD163 antibody 83285-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols