CoraLite® Plus 647 Anti-Human CD163 (GHI/61)
CD163 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
GHI/61
验证数据展示
经过测试的应用
Positive FC detected in | human peripheral blood monocytes |
Planning an IHC experiment? We recommend our IHCeasy CD163 Ready-To-Use IHC Kit. CD163 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65169-1-Ig
推荐稀释比
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-65169 targets CD163 in FC applications and shows reactivity with Human samples.
Tested Applications | FC Application Description |
Tested Reactivity | Human |
Immunogen | Glycoprotein preparation from hairy cell leukemia spleen 种属同源性预测 |
Host / Isotype | Mouse / IgG1, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | CD163 molecule |
Synonyms | CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163 |
Calculated Molecular Weight | 1156 aa, 125 kDa |
GenBank Accession Number | BC051281 |
Gene Symbol | CD163 |
Gene ID (NCBI) | 9332 |
ENSEMBL Gene ID | ENSG00000177575 |
RRID | AB_2883719 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q86VB7 |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 CD163 antibody CL647-65169 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |